HTRA1 promotes EMT through the HDAC6/Ac-α-tubulin pathway in human GBM cells.

ABSTRACT:

Background

The infiltrative nature of human gliomas renders complete surgical removal of tumors futile. Thus, illuminating mechanisms of their infiltrative properties may improve therapies and outcomes of glioma patients.

Methods

Comprehensive bioinformatic analyses of PRSS family were undertaken. Transfection of HTRA1 siRNAs was used to suppress HTRA1 expression. CCK-8, EdU, and colony formation assay were employed to assess cell viability, and cell migration/invasion was detected by transwell, wound healing, and 3D tumor spheroid invasion assays. Immunoprecipitation was applied to study the mechanism that HTRA1 affected cell migration. In addition, in situ xenograft tumor model was employed to explore the role of HTRA1 in glioma growth in vivo.

Results

HTRA1 knockdo

SUBMITTER: Zhao W 

PROVIDER: S-EPMC10853898 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature