Assessment of ATP Metabolism to Adenosine by Ecto-Nucleotidases Carried by Tumor-Derived Small Extracellular Vesicles.

ABSTRACT:

Background

Immunosuppression is a hallmark of cancer progression. Tumor-derived small extracellular vesicles (sEV), also known as TEX, produce adenosine (ADO) and can mediate tumor-induced immunosuppression.

Methods

Here, the ATP pathway of ADO production (ATP◊ADP◊AMP◊ADO) by ecto-nucleotidases carried in sEV was evaluated by a novel method using N⁶-etheno-ATP (eATP) and N⁶-etheno-AMP (eAMP) as substrates. The "downstream" N⁶-etheno-purines (ePurines) were measured by high performance liquid chromatography with fluorescence detection (HPLC-FL).

Results

Human melanoma cell-derived TEX (MTEX) metabolized eATP to N⁶-etheno-ADP (eADP), eAMP and N⁶-etheno-Adenosine (eADO) more robustly than control keratinocyte cell-de

SUBMITTER: Hong CS 

PROVIDER: S-EPMC10854312 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature