The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism.

ABSTRACT:

Context

Jian Gan powder (JGP) is a Chinese medicine compound comprised ginseng, Radix Paeoniae Alba, Radix Astragali, Salvia miltiorrhiza, Yujin, Rhizoma Cyperi, Fructus aurantii, Sophora flavescens, Yinchen, Bupleurum and licorice.

Objective

This study explored the inhibitory effects, polarization and potential mechanisms associated with JGP in macrophages.

Materials and methods

RAW264.7 cells were randomly divided into six groups for 24 h: control, lipopolysaccharide (LPS), overexpression, 1% JGP, 2% JGP, 4% JGP, 8% JGP and 16% JGP. The effects of JGP on RAW264.7 cell proliferation were assessed using colony formation assays and cell counting kit-8 (CCK-8) assays. The Transwell assay was used to evaluate its impact on RAW264.7 cell migration. Moreover, we analysed t

SUBMITTER: Li K 

PROVIDER: S-EPMC10854435 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature