Simplifying Genotyping of Mutants from Genome Editing with a Parallel qPCR-Based iGenotype Index.

ABSTRACT: Targeted genome editing is a powerful tool in reverse genetic studies of gene function in many aspects of biological and pathological processes. The CRISPR/Cas system or engineered endonucleases such as ZFNs and TALENs are the most widely used genome editing tools that are introduced into cells or fertilized eggs to generate double-strand DNA breaks within the targeted region, triggering cellular DNA repair through either homologous recombination or non-homologous end joining (NHEJ). DNA repair through the NHEJ mechanism is usually error-prone, leading to point mutations or indels (insertions and deletions) within the targeted region. Some of the mutations in embryos are germline transmissible, thus providing an effective way to generate model organisms with targeted gene mutations. Howeve

SUBMITTER: Fu L 

PROVIDER: S-EPMC10854663 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature