Near-Infrared In Vivo Imaging of Claudin-1 Expression by Orthotopically Implanted Patient-Derived Colonic Adenoma Organoids.

ABSTRACT:

Background

Claudin-1 becomes overexpressed during the transformation of normal colonic mucosa to colorectal cancer (CRC).

Methods

Patient-derived organoids expressed clinically relevant target levels and genetic heterogeneity, and were established from human adenoma and normal colons. Colonoids were implanted orthotopically in the colon of immunocompromised mice. This pre-clinical model of CRC provides an intact microenvironment and representative vasculature. Colonoid growth was monitored using white light endoscopy. A peptide specific for claudin-1 was fluorescently labeled for intravenous administration. NIR fluorescence images were collected using endoscopy and endomicroscopy.

Results

NIR fluorescence images collected using wide-field endoscopy showed a significan

SUBMITTER: Jaiswal S 

PROVIDER: S-EPMC10854921 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature