Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture.

ABSTRACT: GITDs are among the most common causes of death in adult and young horses in the United States (US). Previous studies have indicated a connection between GITDs and the equine gut microbiome. However, the low taxonomic resolution of the current microbiome sequencing methods has hampered the identification of specific bacterial changes associated with GITDs in horses. Here, we have compared TEHC, a new approach for 16S rRNA gene selection and sequencing, with conventional 16S rRNA gene amplicon sequencing for the characterization of the equine fecal microbiome. Both sequencing approaches were used to determine the fecal microbiome of four adult horses and one commercial mock microbiome. Our results show that TEHC yielded significantly more operational taxonomic units (OTUs) than conventional

SUBMITTER: Alvarez Narvaez S 

PROVIDER: S-EPMC10854943 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature