NSCLC Digital PCR Panel Returns Low-Input Sample Results Where Sequencing Fails.

ABSTRACT: Molecular diagnostics has drastically improved the survival rate of patients diagnosed with non-small cell lung cancer (NSCLC) over the last 10 years. Despite advancements in molecular testing, targeted therapies, and national guideline recommendations, more than half of NSCLC patients in the United States either never receive testing or patient care is not informed via molecular testing. Here, we sought to explore the relationship between DNA/RNA input, the molecular testing method, and test success rates. On a shared set of low-input reference test materials (n = 3), we ran both a hybrid capture-based, next-generation sequencing (NGS) assay and a multiplexed digital PCR (dPCR) panel. The dPCR panel was highly sensitive and specific for low-input samples in dilution studies ranging

SUBMITTER: Herdt LR 

PROVIDER: S-EPMC10854965 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature