Systematic Comparison of Computational Tools for Sanger Sequencing-Based Genome Editing Analysis.

ABSTRACT: Successful genome editing depends on the cleavage efficiency of programmable nucleases (PNs) such as the CRISPR-Cas system. Various methods have been developed to assess the efficiency of PNs, most of which estimate the occurrence of indels caused by PN-induced double-strand breaks. In these methods, PN genomic target sites are amplified through PCR, and the resulting PCR products are subsequently analyzed using Sanger sequencing, high-throughput sequencing, or mismatch detection assays. Among these methods, Sanger sequencing of PCR products followed by indel analysis using online web tools has gained popularity due to its user-friendly nature. This approach estimates indel frequencies by computationally analyzing sequencing trace data. However, the accuracy of these computational tools re

SUBMITTER: Aoki K 

PROVIDER: S-EPMC10854981 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature