Optimization of Protease Treatment Conditions for Chlorella pyrenoidosa Protein Extraction and Investigation of Its Potential as an Alternative Protein Source.

ABSTRACT: This study aimed to determine enzymes that effectively extract Chlorella pyrenoidosa proteins and optimize the processing conditions using response surface methods. Furthermore, the potential of enzymatically hydrolyzed C. pyrenoidosa protein extract (CPE) as a substitute protein source was investigated. The enzymatic hydrolysis conditions for protein extraction were optimized using single-factor analysis and a response surface methodology-Box-Behnken design. The R² value of the optimized model was 0.9270, indicating the reliability of the model, and the optimal conditions were as follows: a hydrolysis temperature of 45.56 °C, pH 9.1, and a hydrolysis time of 49.85 min. The amino acid composition of CPE was compared to that of C. pyrenoidosa powder (CP), whi

SUBMITTER: Cho KJ 

PROVIDER: S-EPMC10855255 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature