The Analysis of Embryoid Body Formation and Its Role in Retinal Organoid Development.

ABSTRACT: Within the last decade, a wide variety of protocols have emerged for the generation of retinal organoids. A subset of studies have compared protocols based on stem cell source, the physical features of the microenvironment, and both internal and external signals, all features that influence embryoid body and retinal organoid formation. Most of these comparisons have focused on the effect of signaling pathways on retinal organoid development. In this study, our aim is to understand whether starting cell conditions, specifically those involved in embryoid body formation, affect the development of retinal organoids in terms of differentiation capacity and reproducibility. To investigate this, we used the popular 3D floating culture method to generate retinal organoids from stem cells. This me

SUBMITTER: Heredero Berzal A 

PROVIDER: S-EPMC10855324 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature