Physical Peculiarity of Two Sites in Human Promoters: Universality and Diverse Usage in Gene Function.

ABSTRACT: Since the discovery of physical peculiarities around transcription start sites (TSSs) and a site corresponding to the TATA box, research has revealed only the average features of these sites. Unsettled enigmas include the individual genes with these features and whether they relate to gene function. Herein, using 10 physical properties of DNA, including duplex DNA free energy, base stacking energy, protein-induced deformability, and stabilizing energy of Z-DNA, we clarified for the first time that approximately 97% of the promoters of 21,056 human protein-coding genes have distinctive physical properties around the TSS and/or position -27; of these, nearly 65% exhibited such properties at both sites. Furthermore, about 55% of the 21,056 genes had a minimum value of regional duplex DNA free

SUBMITTER: Uemura K 

PROVIDER: S-EPMC10855393 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature