Immobilization of Enological Pectinase on Magnetic Sensitive Polyamide Microparticles for Wine Clarification.

ABSTRACT: The use of free pectinases as clarification biocatalysts constitutes a well-established practice in the large-scale production of various types of wines. However, when in the form of free enzymes, the recovery and reusability of pectinases is difficult if not impossible. To address these limitations, the present study focuses on the noncovalent adsorption immobilization of a commercial pectinolytic preparation onto highly porous polyamide 6 (PA6) microparticles, both with and without magnetic properties, prepared via activated anionic polymerization. The two pectinase complexes resulting after immobilization underwent comparative activity and kinetic studies, contrasting them with the free enzyme preparation. In comparison with the free enzyme, the PA6-immobilized pectinase complexes exhib

SUBMITTER: Oliveira SC 

PROVIDER: S-EPMC10855548 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature