Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity Assessment.

ABSTRACT: Three-dimensional (3D) bioprinting is one of the most promising methodologies that are currently in development for the replacement of animal experiments. Bioprinting and most alternative technologies rely on animal-derived materials, which compromises the intent of animal welfare and results in the generation of chimeric systems of limited value. The current study therefore presents the first bioprinted liver model that is entirely void of animal-derived constituents. Initially, HuH-7 cells underwent adaptation to a chemically defined medium (CDM). The adapted cells exhibited high survival rates (85-92%) after cryopreservation in chemically defined freezing media, comparable to those preserved in standard medium (86-92%). Xeno-free bioink for 3D bioprinting yielded liver models with high

SUBMITTER: Ali ASM 

PROVIDER: S-EPMC10855587 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature