Vaccinia Virus Defective Particles Lacking the F17 Protein Do Not Inhibit Protein Synthesis: F17, a Double-Edged Sword for Protein Synthesis?

ABSTRACT: Vaccinia virus (Orthopoxvirus) F17 protein is a major virion structural phosphoprotein having a molecular weight of 11 kDa. Recently, it was shown that F17 synthesised in infected cells interacts with mTOR subunits to evade cell immunity and stimulate late viral protein synthesis. Several years back, we purified an 11 kDa protein that inhibited protein synthesis in reticulocyte lysate from virions, and that possesses all physico-chemical properties of F17 protein. To investigate this discrepancy, we used defective vaccinia virus particles devoid of the F17 protein (designated iF17^- particles) to assess their ability to inhibit protein synthesis. To this aim, we purified iF17^- particles from cells infected with a vaccinia virus mutant which expresses F17 only in

SUBMITTER: Beaud G 

PROVIDER: S-EPMC10855608 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature