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Long-Term Kinetics of SARS-CoV-2 Neutralizing and Anti-Receptor Binding Domain Antibodies among Laboratory-Confirmed COVID-19 Cases in Delhi National Capital Region, India: A Prospective, One-Year Follow-Up Study.

ABSTRACT: Background: This study was conducted with the objective of measuring the neutralizing and anti-receptor binding domain antibody levels against SARS-CoV-2 among laboratory-confirmed COVID-19 cases and exploring its long-term kinetics over a period of 1 year. Methods: One hundred laboratory-confirmed COVID-19 cases were recruited. Serum samples of the participants were collected within three months from the date of the positive COVID-19 report. The participants were prospectively followed up every three months for symptoms and the collection of blood samples for three additional rounds. The presence of anti-SARS-CoV-2 antibodies (IgA, IgG, and IgM antibodies), anti-receptor binding domain antibodies (anti-RBD), and neutralizing antibodies were measured. Findings: Median plaque reduction neutralization test (PRNT) titers showed a rising trend in the first three rounds of follow-up. The quantitative anti-receptor binding domain ELISA (QRBD) values showed a declining trend in the initial three rounds. However, both the PRNT titers and QRBD values showed significantly higher values for the fourth round of follow-up. Total antibody (WANTAI) levels showed an increasing trend in the initial three rounds (statistically significant). Interpretation: Neutralizing antibodies showed an increasing trend. The anti-receptor binding domain antibodies showed a decreasing trend. Neutralizing antibodies and anti-RBD antibodies persisted in the majority.

SUBMITTER: Misra P

PROVIDER: S-EPMC10856624 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Long-Term Kinetics of SARS-CoV-2 Neutralizing and Anti-Receptor Binding Domain Antibodies among Laboratory-Confirmed COVID-19 Cases in Delhi National Capital Region, India: A Prospective, One-Year Follow-Up Study.

Misra Puneet P Medigeshi Guruprasad R GR Kant Shashi S Jaiswal Abhishek A Ahmad Mohammad M Rahman Anisur A Guleria Randeep R Rai Sanjay Kumar SK Deori Trideep Jyoti TJ Mandal Suprakash S Gongal Gaurav G Bairwa Mohan M Haldar Partha P Kumar Rakesh R Garg Neha N

Journal of clinical medicine 20240129 3

<b>Background:</b> This study was conducted with the objective of measuring the neutralizing and anti-receptor binding domain antibody levels against SARS-CoV-2 among laboratory-confirmed COVID-19 cases and exploring its long-term kinetics over a period of 1 year. <b>Methods:</b> One hundred laboratory-confirmed COVID-19 cases were recruited. Serum samples of the participants were collected within three months from the date of the positive COVID-19 report. The participants were prospectively fol ...[more]

PMID: 38337457

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Project description:BackgroundMiltefosine unresponsive and relapse cases of visceral leishmaniasis (VL) are increasingly being reported. However, there has been no laboratory confirmed reports of miltefosine resistance in VL. Here, we report two laboratory confirmed cases of VL from India.MethodsTwo patients with VL were referred to us with suspected VL. The first patient was a native of the VL endemic state of Bihar, but residing in Delhi, a VL non-endemic area. He was treated with broad-spectrum antibiotics and antipyretics but was unresponsive to treatment. The second patient was from Jharkhand state in eastern India (adjoining Bihar), another endemic state for VL. He was refractory to anti-leishmanial treatment, which included administration of miltefosine. Following investigation, both patients were serologically positive for VL, and blood buffy coat from both patients grew Leishmania donovani. The isolates derived from both cases were characterized for their drug susceptibility, genetically characterised, and SNPs typed for LdMT and LdROS gene expression. Both patients were successfully treated with amphotericin B.ResultsThe in vitro drug susceptibility assays carried out on both isolates showed good IC50 values to amphotericin B (0.1 ± 0.0004 μg/ml and 0.07 ± 0.0019 μg/ml). One isolate was refractory to SbIII with an IC50 of > 200 μM while the second isolate was sensitive to SbIII with an IC50 of 36.70 ± 3.2 μM. However, in both the isolates, IC50 against miltefosine was more than 10-fold higher (> 100 μM) than the standard strain DD8 (6.8 ± 0.1181 μM). Furthermore, genetic analyses demonstrated single nucleotide polymorphisms (SNPs) (354Tyr↔Phe and 1078Phe↔Tyr) in the LdMT gene of the parasites.ConclusionsHere, we document two laboratory confirmed cases of miltefosine resistant VL from India. Our finding highlights the urgent need to establish control measures to prevent the spread of these strains. We also propose that LdMT gene mutation analysis could be used as a molecular marker of miltefosine resistance in L. donovani.

Project description:Globally, since the end of December 2019, coronavirus disease (COVID-19) has been recognized as a severe infectious disease. Therefore, this study has been attempted to examine the linkage between climatic variables and COVID-19 particularly in National Capital Territory of Delhi (NCT of Delhi), India. For this, daily data of COVID-19 has been used for the period March 14 to June 11, 2020, (90 days). Eight climatic variables such as maximum, minimum and mean temperature (°C), relative humidity (%), bright sunshine hours, wind speed (km/h), evaporation (mm), and rainfall (mm) have been analyzed in relation to COVID-19. To study the relationship among different climatic variables and COVID-19 spread, Karl Pearson’s correlation analysis has been performed. The Mann–Kendall method and Sen’s slope estimator have been used to detect the direction and magnitude of COVID-19 trends, respectively. The results have shown that out of eight selected climatic variables, six variables, viz. maximum temperature, minimum temperature, mean temperature, relative humidity, evaporation, and wind speed are positively associated with coronavirus disease cases (statistically significant at 95 and 99% confidence levels). No association of coronavirus disease has been found with bright sunshine hours and rainfall. Besides, COVID-19 cases and deaths have shown increasing trends, significant at 99% confidence level. The results of this study suggest that climatic conditions in NCT of Delhi are favorable for COVID-19 and the disease may spread further with the increasing temperature, relative humidity, evaporation and wind speed. This is the only study which has presented the analysis of COVID-19 spread in relation to several climatic variables for the most densely populated and rapidly growing city of India. Thus, considering the results obtained, effective policies and actions are necessary especially by identifying the areas where the spread rate is increasing rapidly in this megacity. The prevention and protection measures should be adopted aiming at to reduce the further transmission of disease in the city.

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Dataset Information

Long-Term Kinetics of SARS-CoV-2 Neutralizing and Anti-Receptor Binding Domain Antibodies among Laboratory-Confirmed COVID-19 Cases in Delhi National Capital Region, India: A Prospective, One-Year Follow-Up Study.

Publications

Long-Term Kinetics of SARS-CoV-2 Neutralizing and Anti-Receptor Binding Domain Antibodies among Laboratory-Confirmed COVID-19 Cases in Delhi National Capital Region, India: A Prospective, One-Year Follow-Up Study.

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