New Prospects for Improving Microspore Embryogenesis Induction in Highly Recalcitrant Winter Wheat Lines.

ABSTRACT: Among various methods stimulating biological progress, double haploid (DH) technology, which utilizes the process of microspore embryogenesis (ME), is potentially the most effective. However, the process depends on complex interactions between many genetic, physiological and environmental variables, and in many cases, e.g., winter wheat, does not operate with the efficiency required for commercial use. Stress associated with low-temperature treatment, isolation and transfer to in vitro culture has been shown to disturb redox homeostasis and generate relatively high levels of reactive oxygen species (ROS), affecting microspore vitality. The aim of this study was to investigate whether controlled plant growth, specific tiller pre-treatment and culture conditions could improve the potential o

SUBMITTER: Dubas E 

PROVIDER: S-EPMC10857491 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature