Nanopore Direct RNA Sequencing Reveals the Short-Term Salt Stress Response in Maize Roots.

ABSTRACT: Transcriptome analysis, relying on the cutting-edge sequencing of cDNA libraries, has become increasingly prevalent within functional genome studies. However, the dependence on cDNA in most RNA sequencing technologies restricts their ability to detect RNA base modifications. To address this limitation, the latest Oxford Nanopore Direct RNA Sequencing (ONT DRS) technology was employed to investigate the transcriptome of maize seedling roots under salt stress. This approach aimed to unveil both the RNA transcriptional profiles and alterations in base modifications. The analysis of the differential expression revealed a total of 1398 genes and 2223 transcripts that exhibited significant variation within the maize root system following brief exposure to salt stress. Enrichment analyses, such a

SUBMITTER: He S 

PROVIDER: S-EPMC10857558 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature