An Integrated Approach to Improve the Assay Performance of Quantum Dot-Based Lateral Flow Immunoassays by Using Silver Deposition.

ABSTRACT: Traditional quantum dot-based lateral flow immunoassay (QD-LFIA) is limited to signal loss in part by the blinking, photobleaching and oxidative quenching of QD probes. Inspired by the good application of silver deposition on QD surfaces in tissue imaging, and in the context of improving the assay performance without compromising the simplicity and practicality, we report that introducing the QD-silver combination to the LFIA system, has the advantages of accuracy improvement, signal enhancement and user friendliness promotion, but maintains the cost-effective property and commercial accessibility of QD-LFIA. The effect was shown by using CdSe/ZnS QD-LFIA coupled with anti-sodium pentachlorophenate antibody, which provided a 4-fold improvement in the signal, a 2.5-fold improvement in the d

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10857874 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature