Anti-Toxoplasma gondii effect of tylosin in vitro and in vivo.

ABSTRACT:

Background

Toxoplasma gondii is an important protozoan pathogen with medical and veterinary importance worldwide. Drugs currently used for treatment of toxoplasmosis are less effective and sometimes cause serious side effects. There is an urgent need for the development of more effective drugs with relatively low toxicity.

Methods

The effect of tylosin on the viability of host cells was measured using CCK8 assays. To assess the inhibition of tylosin on T. gondii proliferation, a real-time PCR targeting the B1 gene was developed for T. gondii detection and quantification. Total RNA was extracted from parasites treated with tylosin and then subjected to transcriptome analysis by RNA sequencing (RNA-seq). Finally, murine infection models of toxoplasmosis were used to evaluate t

SUBMITTER: Han RX 

PROVIDER: S-EPMC10858492 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature