Enhanced molecular release from elderly bone samples using collagenase I: insights into fatty acid metabolism alterations.

ABSTRACT:

Background

Bone is a metabolically active tissue containing different cell types acting as endocrine targets and effectors. Further, bone is a dynamic depot for calcium, phosphorous and other essential minerals. The tissue matrix is subjected to a constant turnover in response to mechanical/endocrine stimuli. Bone turnover demands high energy levels, making fatty acids a crucial source for the bone cells. However, the current understanding of bone cell metabolism is poor. This is partly due to bone matrix complexity and difficulty in small molecules extraction from bone samples. This study aimed to evaluate the effect of metabolite sequestering from a protein-dominated matrix to increase the quality and amount of metabolomics data in discovering small molecule patterns in pathologi

SUBMITTER: Malvandi AM 

PROVIDER: S-EPMC10858523 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature