Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells.

ABSTRACT: Human cerebral organoids (hCOs) offer the possibility of deepening the knowledge of human brain development, as well as the pathologies that affect it. The method developed here describes the efficient generation of hCOs by going directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures to three-dimensional (3D) neuroepithelial tissue, avoiding dissociation and aggregation steps. This has been achieved by subjecting 2D cultures, from the beginning of the neural induction step, to dual-SMAD inhibition in combination with CHIR99021. This is a simple and reproducible protocol in which the hCOs generated develop properly presenting proliferative ventricular zones (VZs) formed by neural precursor and radial glia (RG) that differentiate to give rise to mature neurons and glial cell

SUBMITTER: Gonzalez-Sastre R 

PROVIDER: S-EPMC10858658 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature