Facilitating long-term cell examinations and time-lapse recordings in cell biology research with CO₂ mini-incubators.

ABSTRACT: In recent years, microscopy has revolutionized the study of dynamic living cells. However, performing long-term live cell imaging requires stable environmental conditions such as temperature, pH, and humidity. While standard incubators have traditionally provided these conditions, other solutions, like stagetop incubators are available. To further enhance the accessibility of stable cell culture environments for live cell imaging, we developed a portable CO₂ cell culture mini-incubator that can be easily adapted to any x-y inverted microscope stage, enabling long-term live cell imaging. This mini-incubator provides and maintains stable environmental conditions and supports cell viability comparable to standard incubators. Moreover, it allows for parallel experiments in the same

SUBMITTER: Talebipour A 

PROVIDER: S-EPMC10858865 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature