Pick-up single-cell proteomic analysis for quantifying up to 3000 proteins in a Mammalian cell.

ABSTRACT: The shotgun proteomic analysis is currently the most promising single-cell protein sequencing technology, however its identification level of ~1000 proteins per cell is still insufficient for practical applications. Here, we develop a pick-up single-cell proteomic analysis (PiSPA) workflow to achieve a deep identification capable of quantifying up to 3000 protein groups in a mammalian cell using the label-free quantitative method. The PiSPA workflow is specially established for single-cell samples mainly based on a nanoliter-scale microfluidic liquid handling robot, capable of achieving single-cell capture, pretreatment and injection under the pick-up operation strategy. Using this customized workflow with remarkable improvement in protein identification, 2449-3500, 2278-3257 and 1621-2904

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10858870 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature