Aspartyl protease in the secretome of honey bee trypanosomatid parasite contributes to infection of bees.

ABSTRACT:

Background

The exoproteome, which consists of both secreted proteins and those originating from cell surfaces and lysed cells, is a critical component of trypanosomatid parasites, facilitating interactions with host cells and gut microbiota. However, its specific roles in the insect hosts of these parasites remain poorly understood.

Methods

We conducted a comprehensive characterization of the exoproteome in Lotmaria passim, a trypanosomatid parasite infecting honey bees, under culture conditions. We further investigated the functions of two conventionally secreted proteins, aspartyl protease (LpAsp) and chitinase (LpCht), as representative models to elucidate the role of the secretome in L. passim infection of honey bees.

Results

Approximately 48% of L. passim exoprot

SUBMITTER: Yuan X 

PROVIDER: S-EPMC10859015 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature