Lung single-cell RNA profiling reveals response of pulmonary capillary to sepsis-induced acute lung injury.

ABSTRACT:

Background

Sepsis-induced acute lung injury (ALI) poses a significant threat to human health. Endothelial cells, especially pulmonary capillaries, are the primary barriers against sepsis in the lungs. Therefore, investigating endothelial cell function is essential to understand the pathophysiological processes of sepsis-induced ALI.

Methods

We downloaded single-cell RNA-seq expression data from GEO with accession number GSE207651. The mice underwent cecal ligation and puncture (CLP) surgery, and lung tissue samples were collected at 0, 24, and 48 h. The cells were annotated using the CellMarker database and FindAllMarkers functions. GO enrichment analyses were performed using the Metascape software. Gene set enrichment Analysis (GSEA) and variation Analysis (GSVA) were perfo

SUBMITTER: Yang R 

PROVIDER: S-EPMC10859485 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature