Enhancing dendrobine production in Dendrobium nobile through mono-culturing of endophytic fungi, Trichoderma longibrachiatum (MD33) in a temporary immersion bioreactor system.

ABSTRACT:

Introduction

Dendrobine, a valuable alkaloid found in Dendrobium nobile, possesses significant pharmaceutical potential.

Methods

In this study, we explored innovative approaches to enhance dendrobine production by utilizing endophytic fungi in a Temporary Immersion Bioreactor System (TIBS, Nanjing BioFunction Co. Ltd., China) and traditional test bottles. Dendrobine was unequivocally identified and characterised in D. nobile co-culture seedlings through UHPLC analysis and LC-MS qTOF analysis, supported by reference standards.

Results

The CGTB (control group) and EGTB (experimental group) 12-month-old D. nobile seedlings exhibited similar peak retention times at 7.6±0.1 minutes, with dendrobine identified as C₁₆H₂₅NO₂

SUBMITTER: Sarsaiya S 

PROVIDER: S-EPMC10859523 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature