Comparison of Isolated Lymphocyte and Whole Blood-Based CBMN Assays for Radiation Triage.

ABSTRACT: Following a mass-casualty nuclear/radiological event, there will be an important need for rapid and accurate estimation of absorbed dose for biological triage. The cytokinesis-block micronucleus (CBMN) assay is an established and validated cytogenetic biomarker used to assess DNA damage in irradiated peripheral blood lymphocytes. Here, we describe an intercomparison experiment between two biodosimetry laboratories, located at Columbia University (CU) and Health Canada (HC) that performed different variants of the human blood CBMN assay to reconstruct dose in human blood, with CU performing the assay on isolated lymphocytes and using semi-automated scoring whereas HC used the more conventional whole blood assay. Although the micronucleus yields varied significantly between the two assays, t

SUBMITTER: Bertucci A 

PROVIDER: S-EPMC10859551 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature