Enhancing protein dynamics analysis with hydrophilic polyethylene glycol cross-linkers.

ABSTRACT: Cross-linkers play a critical role in capturing protein dynamics in chemical cross-linking mass spectrometry techniques. Various types of cross-linkers with different backbone features are widely used in the study of proteins. However, it is still not clear how the cross-linkers' backbone affect their own structure and their interactions with proteins. In this study, we systematically characterized and compared methylene backbone and polyethylene glycol (PEG) backbone cross-linkers in terms of capturing protein structure and dynamics. The results indicate the cross-linker with PEG backbone have a better ability to capture the inter-domain dynamics of calmodulin, adenylate kinase, maltodextrin binding protein and dual-specificity protein phosphatase. We further conducted quantum chemical ca

SUBMITTER: Sun M 

PROVIDER: S-EPMC10859660 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature