Standardizing In Vitro β-Lactam Antibiotic Allergy Testing with Synthetic IgE.

ABSTRACT: The global prevalence of β-lactam allergy poses a major challenge in administering first-line antibiotics, such as penicillins, to a significant portion of the population. The lack of β-lactam IgE antibody pools with defined selectivity hampers the standardization and validation of in vitro assays for β-lactam allergy testing. To address this limitation, this study introduces a synthetic IgE specific to β-lactam antibiotics as a valuable tool for drug allergy research and diagnostic tests. Using phage display technology, we constructed a library of human single-chain antibody fragments (scFv) to target the primary determinant of amoxicillin, a widely used β-lactam antibiotic. Subsequently, we produced a complete human synthetic IgE molecule using the highly efficient baculovirus expression

SUBMITTER: Quintero-Campos P 

PROVIDER: S-EPMC10859892 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature