M2 macrophages promote subconjunctival fibrosis through YAP/TAZ signalling.

ABSTRACT:

Purpose

To evaluate the role of M2 macrophages in subconjunctival fibrosis after silicone implantation (SI) and investigate the underlying mechanisms.

Materials and methods

A model of subconjunctival fibrosis was established by SI surgery in rabbit eyes. M2 distribution and collagen deposition were evaluated by histopathology. The effects of M2 cells on the migration (using wound-scratch assay) and activation (by immunofluorescence and western blotting) of human Tenon's fibroblasts (HTFs) were investigated.

Results

There were more M2 macrophages (CD68+/CD206+ cells) occurring in tissue samples around silicone implant at 2 weeks postoperatively. Dense collagen deposition was observed at 8 weeks after SI. In vitro experiment showed M2 expressed high level of CD20

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10860428 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature