Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects.

ABSTRACT:

Purpose

To compare regional conjunctival expression of membrane-associated mucins (MAMs) MUC1, MUC4, and MUC16 in normal and dry eye (DE) subjects.

Methods

Adults with and without signs and symptoms of DE were recruited. Impression cytology was performed to collect MAMs from four bulbar and upper eyelid palpebral conjunctival regions of both eyes. After protein extraction, samples from both eyes of a single subject were pooled by region, and expression was analyzed using a capillary electrophoresis nano-immunoassay system. The chemiluminescence intensity of each antigen binding signal was calculated after normalization to the total protein amount. Statistical analyses were conducted using GraphPad Prime 9.

Results

Samples from thirteen to sixteen DE and seven to eleve

SUBMITTER: Choi M 

PROVIDER: S-EPMC10860684 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature