Hydrogen Sulfide Producers Drive a Diarrhea-Like Phenotype and a Methane Producer Drives a Constipation-Like Phenotype in Animal Models.

ABSTRACT:

Background

We recently demonstrated that diarrhea-predominant irritable bowel syndrome (IBS-D) subjects have higher relative abundance (RA) of hydrogen sulfide (H₂S)-producing Fusobacterium and Desulfovibrio species, and constipation-predominant IBS (IBS-C) subjects have higher RA of methanogen Methanobrevibacter smithii.

Aims

In this study, we investigate the effects of increased methanogens or H₂S producers on stool phenotypes in rat models.

Methods

Adult Sprague-Dawley rats were fed high-fat diet (HFD) for 60 days to increase M. smithii levels, then gavaged for 10 days with water (controls) or methanogenesis inhibitors. To increase H₂S producers, rats were gavaged with F. varium or D. piger. Stool consistency (stool wet weight (SWW)

SUBMITTER: Villanueva-Millan MJ 

PROVIDER: S-EPMC10861391 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature