HS-GC-MS analysis of volatile organic compounds after hyperoxia-induced oxidative stress: a validation study.

ABSTRACT:

Background

Exhaled volatile organic compounds (VOCs), particularly hydrocarbons from oxidative stress-induced lipid peroxidation, are associated with hyperoxia exposure. However, important heterogeneity amongst identified VOCs and concerns about their precise pathophysiological origins warrant translational studies assessing their validity as a marker of hyperoxia-induced oxidative stress. Therefore, this study sought to examine changes in VOCs previously associated with the oxidative stress response in hyperoxia-exposed lung epithelial cells.

Methods

A549 alveolar epithelial cells were exposed to hyperoxia for 24 h, or to room air as normoxia controls, or hydrogen peroxide as oxidative-stress positive controls. VOCs were sampled from the headspace, analysed by gas chromatog

SUBMITTER: Lilien TA 

PROVIDER: S-EPMC10861410 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature