The transcriptional regulatory network modulating human trophoblast stem cells to extravillous trophoblast differentiation.

ABSTRACT: During human pregnancy, extravillous trophoblasts play crucial roles in placental invasion into the maternal decidua and spiral artery remodeling. However, regulatory factors and their action mechanisms modulating human extravillous trophoblast specification have been unknown. By analyzing dynamic changes in transcriptome and enhancer profile during human trophoblast stem cell to extravillous trophoblast differentiation, we define stage-specific regulators, including an early-stage transcription factor, TFAP2C, and multiple late-stage transcription factors. Loss-of-function studies confirm the requirement of all transcription factors identified for adequate differentiation, and we reveal that the dynamic changes in the levels of TFAP2C are essential. Notably, TFAP2C pre-occupies the regula

SUBMITTER: Kim M 

PROVIDER: S-EPMC10861538 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature