3D visualization of the human anterior cruciate ligament combining micro-CT and histological analysis.

ABSTRACT:

Purpose

The study aimed to obtain a comprehensive 3D visualization of knee specimens, including the cruciate ligaments and corresponding femoral and tibial bone insertions using a non-destructive micro-CT method.

Methods

Knee specimens were fixed in anatomical positions and chemically dehydrated before being scanned using micro-CT with a voxel size of 17.5 μm. RGBA (red, green, blue, alpha) transfer functions were applied to virtually colorize each structure. Following micro-CT scanning, the samples were rehydrated, decalcified, and trimmed based on micro-CT 3D reconstructions as references. Histological evaluations were performed on the trimmed samples. Histological and micro-CT images were registered to morphologically and densitometrically assess the 4-layer insertion of

SUBMITTER: Parrilli A 

PROVIDER: S-EPMC10861685 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature