Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains.

ABSTRACT: Neurotransmitter receptors are essential components of synapses for communication between neurons in the brain. Because the spatiotemporal expression profiles and dynamics of neurotransmitter receptors involved in many functions are delicately governed in the brain, in vivo research tools with high spatiotemporal resolution for receptors in intact brains are highly desirable. Covalent labeling by chemical reaction (chemical labeling) of proteins without genetic manipulation is now a powerful method for analyzing receptors in vitro. However, selective target receptor labeling in the brain has not yet been achieved. This study shows that ligand-directed alkoxyacylimidazole (LDAI) chemistry can be used to selectively tether synthetic probes to target endogenous receptors in living mouse brain

SUBMITTER: Nonaka H 

PROVIDER: S-EPMC10861872 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature