Systematic analysis of nonprogrammed frameshift suppression in E. coli via translational tiling proteomics.

ABSTRACT: The synthesis of proteins as encoded in the genome depends critically on translational fidelity. Nevertheless, errors inevitably occur, and those that result in reading frame shifts are particularly consequential because the resulting polypeptides are typically nonfunctional. Despite the generally maladaptive impact of such errors, the proper decoding of certain mRNAs, including many viral mRNAs, depends on a process known as programmed ribosomal frameshifting. The fact that these programmed events, commonly involving a shift to the -1 frame, occur at specific evolutionarily optimized "slippery" sites has facilitated mechanistic investigation. By contrast, less is known about the scope and nature of error (i.e., nonprogrammed) frameshifting. Here, we examine error frameshifting by monitori

SUBMITTER: Springstein BL 

PROVIDER: S-EPMC10861913 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature