Anti-Helicobacter pylori, anti-biofilm activity, and molecular docking study of citropten, bergapten, and its positional isomer isolated from Citrus sinensis L. leaves.

ABSTRACT:

Introduction

Citrus sinensis L. is a candidate plant with promising antimicrobial potential. In the current study, the phytochemical investigation of C. sinensis leaf extract led to the isolation of three coumarins, namely bergapten, xanthotoxin, and citropten.

Methods

The chemical structures of the isolated coumarins were elucidated using NMR and ESI-MS techniques. The total aqueous ethanol leaf extract and the isolated coumarins were evaluated for their antimicrobial effects against Helicobacter pylori using the MTT-micro-well dilution method and its anti-biofilm activity using MBEC assay, as compared to clarithromycin.

Results

The results showed that citropten scored the lowest MIC value at 3.9 μg/mL and completely inhibited the planktonic grow

SUBMITTER: Mohammed HS 

PROVIDER: S-EPMC10861955 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature