TLR9-independent CD8⁺ T cell responses in hepatic AAV gene transfer through IL-1R1-MyD88 signaling.

ABSTRACT: Upon viral infection of the liver, CD8⁺ T cell responses may be triggered despite the immune suppressive properties that manifest in this organ. We sought to identify pathways that activate responses to a neoantigen expressed in hepatocytes, using adeno-associated viral (AAV) gene transfer. It was previously established that cooperation between plasmacytoid dendritic cells (pDCs), which sense AAV genomes by Toll-like receptor 9 (TLR9), and conventional DCs promotes cross-priming of capsid-specific CD8⁺ T cells. Surprisingly, we find local initiation of a CD8⁺ T cell response against antigen expressed in ∼20% of murine hepatocytes, independent of TLR9 or type I interferons and instead relying on IL-1 receptor 1-MyD88 signaling. Both IL-1α and IL-1β contribut

SUBMITTER: Kumar SRP 

PROVIDER: S-EPMC10861967 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature