Generation of antibodies to an extracellular region of the transporters Glut1/Glut4 by immunization with a designed antigen.

ABSTRACT: Monoclonal antibodies are one of the fastest growing class of drugs. Nevertheless, relatively few biologics target multispanning membrane proteins because of technical challenges. To target relatively small extracellular regions of multiple membrane-spanning proteins, synthetic peptides, which are composed of amino acids corresponding to an extracellular region of a membrane protein, are often utilized in antibody discovery. However, antibodies to these peptides often do not recognize parental membrane proteins. In this study, we designed fusion proteins in which an extracellular helix of the membrane protein glucose transporter 1 (Glut1) was grafted onto the scaffold protein Adhiron. In the initial design, the grafted fragment did not form a helical conformation. Molecular dynamics simula

SUBMITTER: Sumikawa T 

PROVIDER: S-EPMC10862009 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature