Tlr5 deficiency exacerbates lupus-like disease in the MRL/lpr mouse model.

ABSTRACT:

Introduction

Leaky gut has been linked to autoimmune disorders including lupus. We previously reported upregulation of anti-flagellin antibodies in the blood of lupus patients and lupus-prone mice, which led to our hypothesis that a leaky gut drives lupus through bacterial flagellin-mediated activation of toll-like receptor 5 (TLR5).

Methods

We created MRL/lpr mice with global Tlr5 deletion through CRISPR/Cas9 and investigated lupus-like disease in these mice.

Result

Contrary to our hypothesis that the deletion of Tlr5 would attenuate lupus, our results showed exacerbation of lupus with Tlr5 deficiency in female MRL/lpr mice. Remarkably higher levels of proteinuria were observed in Tlr5 ^-/- MRL/lpr mice sugge

SUBMITTER: Alajoleen RM 

PROVIDER: S-EPMC10862078 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature