Interfacial Electromigration for Analysis of Biofluid Lipids in Small Volumes.

ABSTRACT: Lipids are important biomarkers within the field of disease diagnostics and can serve as indicators of disease progression and predictors of treatment effectiveness. Although lipids can provide important insight into how diseases initiate and progress, mass spectrometric methods for lipid characterization and profiling are limited due to lipid structural diversity, particularly the presence of various lipid isomers. Moreover, the difficulty of handling small-volume samples exacerbates the intricacies of biological analyses. In this work, we have developed a strategy that electromigrates a thin film of a small-volume biological sample directly to the air-liquid interface formed at the tip of a theta capillary. Importantly, we seamlessly integrated in situ biological lipid extraction with ac

SUBMITTER: Edwards ME 

PROVIDER: S-EPMC10862378 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature