Cross talk between the liver microbiome and epigenome in patients with metabolic dysfunction-associated steatotic liver disease.

ABSTRACT:

Background

The pathogenesis of MASLD (metabolic dysfunction-associated steatotic liver disease), including its severe clinical forms, involves complex processes at all levels of biological organization. This study examined the potential link between the liver microbiome profile and epigenetic factors.

Methods

Liver microbial DNA composition was analysed using high throughput 16S rRNA gene sequencing in 116 individuals, with 55% being female, across the spectrum of liver disease severity. Total activity of histone deacetylases (HDACs) and acetyltransferases (HATs) was assayed in nuclear extracts from fresh liver samples. In addition, we measured the global 5-hydroxymethylcytosine (5-hmC) levels of liver DNA.

Findings

Patients with MASLD showed a 2.07-fold increase (p =

SUBMITTER: Pirola CJ 

PROVIDER: S-EPMC10862506 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature