Cortical acetylcholine dynamics are predicted by cholinergic axon activity and behavior state.

ABSTRACT: Even under spontaneous conditions and in the absence of changing environmental demands, awake animals alternate between increased or decreased periods of alertness. These changes in brain state can occur rapidly, on a timescale of seconds, and neuromodulators such as acetylcholine (ACh) are thought to play an important role in driving these spontaneous state transitions. Here, we perform the first simultaneous imaging of ACh sensors and GCaMP-expressing axons in vivo, to examine the spatiotemporal properties of cortical ACh activity and release during spontaneous changes in behavioral state. We observed a high correlation between simultaneously recorded basal forebrain axon activity and neuromodulator sensor fluorescence around periods of locomotion and pupil dilation. Consistent wi

SUBMITTER: Neyhart E 

PROVIDER: S-EPMC10862699 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature