Interferon-γ signaling in eosinophilic esophagitis has implications for epithelial barrier function and programmed cell death.

ABSTRACT:

Objective

Eosinophilic esophagitis (EoE) is a chronic esophageal inflammatory disorder characterized by eosinophil-rich mucosal inflammation and tissue remodeling. Transcriptional profiling of esophageal biopsies has previously revealed upregulation of type I and II interferon (IFN) response genes. We aim to unravel interactions between immune and epithelial cells and examine functional significance in esophageal epithelial cells.

Design

We investigated epithelial gene expression from EoE patients using single-cell RNA sequencing and a confirmatory bulk RNA-sequencing experiment of isolated epithelial cells. The functional impact of interferon signaling on epithelial cells was investigated using in vitro organoid models.

Results

We observe upregulation of inter

SUBMITTER: Lal M 

PROVIDER: S-EPMC10862711 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature