MiR-26 deficiency causes alterations in lens transcriptome and results in adult-onset cataract.

ABSTRACT:

Purpose

Despite strong evidence demonstrating that normal lens development requires regulation governed by miRNAs, the functional role of specific miRNAs in mammalian lens development remains largely unexplored.

Methods

A comprehensive analysis of miRNA transcripts in the newborn mouse lens, exploring both differential expression between lens epithelial cells and lens fiber cells and overall miRNA abundance was conducted by miRNA-seq. Mouse lenses lacking each of three abundantly expressed lens miRNAs: miR-184, miR-26 and miR-1 were analyzed to explore the role of these miRNAs in lens development.

Results

Mice lacking all three copies of miR-26 (miR-26^TKO) developed postnatal cataracts as early as 4-6 weeks of age. RNA-seq analysis of n

SUBMITTER: Upreti A 

PROVIDER: S-EPMC10862774 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature