Mendelian segregation and high recombination rates facilitate genetic analyses in Cryptosporidium parvum.

ABSTRACT: Very little is known about the process of meiosis in the apicomplexan parasite Cryptosporidium despite the essentiality of sex in its life cycle. Most cell lines only support asexual growth of Cryptosporidium parvum (C. parvum), but stem cell derived intestinal epithelial cells grown under air-liquid interface (ALI) conditions support the sexual cycle. To examine chromosomal dynamics during meiosis in C. parvum, we generated two transgenic lines of parasites that were fluorescently tagged with mCherry or GFP on chromosomes 1 or 5, respectively. Infection of ALI cultures or Ifngr1^-/- mice with mCherry and GFP parasites produced "yellow" oocysts generated by cross-fertilization. Outcrossed oocysts from the F₁ generation were pur

SUBMITTER: Kimball A 

PROVIDER: S-EPMC10862819 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature