Structure-guided engineering of a fast genetically encoded sensor for real-time H₂O₂ monitoring.

ABSTRACT: Hydrogen Peroxide (H₂O₂) is a central oxidant in redox biology due to its pleiotropic role in physiology and pathology. However, real-time monitoring of H₂O₂ in living cells and tissues remains a challenge. We address this gap with the development of an optogenetic hydRogen perOxide Sensor (oROS), leveraging the bacterial peroxide binding domain OxyR. Previously engineered OxyR-based fluorescent peroxide sensors lack the necessary sensitivity or response speed for effective real-time monitoring. By structurally redesigning the fusion of Escherichia coli (E. coli) ecOxyR with a circularly permutated green fluorescent protein (cpGFP), we created a novel, green-fluorescent peroxide sensor oROS-G. oROS-G exhibits high sensitivity and fast on-and-off

SUBMITTER: Lee JD 

PROVIDER: S-EPMC10862829 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature