Investigating low frequency somatic mutations in Arabidopsis with Duplex Sequencing.

ABSTRACT: Mutations are the source of novel genetic diversity but can also lead to disease and maladaptation. The conventional view is that mutations occur randomly with respect to their environment-specific fitness consequences. However, intragenomic mutation rates can vary dramatically due to transcription coupled repair and based on local epigenomic modifications, which are non-uniformly distributed across genomes. One sequence feature associated with decreased mutation is higher expression level, which can vary depending on environmental cues. To understand whether the association between expression level and mutation rate creates a systematic relationship with environment-specific fitness effects, we perturbed expression through a heat treatment in Arabidopsis thaliana. We quantified gen

SUBMITTER: Waneka G 

PROVIDER: S-EPMC10862904 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature