Deciphering regulatory architectures from synthetic single-cell expression patterns.

ABSTRACT: For the vast majority of genes in sequenced genomes, there is limited understanding of how they are regulated. Without such knowledge, it is not possible to perform a quantitative theory-experiment dialogue on how such genes give rise to physiological and evolutionary adaptation. One category of high-throughput experiments used to understand the sequence-phenotype relationship of the transcriptome is massively parallel reporter assays (MPRAs). However, to improve the versatility and scalability of MPRA pipelines, we need a "theory of the experiment" to help us better understand the impact of various biological and experimental parameters on the interpretation of experimental data. These parameters include binding site copy number, where a large number of specific binding sites may titrate

SUBMITTER: Pan RW 

PROVIDER: S-EPMC10862939 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature